In-vitro antioxidant activity of hot aqueous extract of Helicteres isora Linn. fruits

The antioxidant activity of the aqueous (hot) extract of Helicteres isora Linn. (AEHI) (Family—Sterculiaceae) fruits was investigated in various in vitro models. The total polyphenolic content of the extract was found 7.04% of AEHI, when compared to gallic acid and total flavonoids content was 2.4 mg/g of AEHI, when compared to rutin. Hydrogen peroxide radicals were inhibited at IC 50 =165 μg/ml, while ascorbic acid inhibited at 187.33 μg/ml. AEHI inhibited the nitric oxide radical at IC 50 = 820 μg/ml, when it was compared with rutin as standard antioxidant with IC 50 = 68.52 μg/ml. Superoxide (SO) radical’s inhibition was compared with quercetin and IC 50 value was found more than 1000 μg/ml. Moreover, the results were observed in a concentration dependent manner. Present in vitro studies clearly indicated that the aqueous (hot) extract of H. isora has significant antioxidant activity.